Oligo Design

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Zebrafish contract research and commercial licenses

For contract research using Morpholinos in zebrafish or to license Morpholinos for commercial use in zebrafish in the USA, contact Zygogen, Inc. See the Outside Resources box for more information.

Use this email form to request FREE design of a Morpholino Antisense Oligo. Neither traffic to and from this website, nor the email that will be generated, is secured so confidential inquiries are more safely conducted by phone or fax.

Fields in marked with an asterisk (*) are required.

This form is only for oligo design. This is NOT an order form. To place an order, use our online store (see the column on the left). To contact Gene Tools for any other reason, please use our Contact Form.

To use Gene Tools' free design service:

A. For translational blocking oligos, provide a GenBank or EMBL accession number for your sequence, OR provide the actual 5' UTR sequence through the 25th base of the coding region. Please mark the start codon with parenthesis ( ) and provide ONLY sequence upstream of the 25th base of coding sequence. Sequence downstream of the 25th base can not be used for translational blocking.

B. For splice blocking oligos, please provide 50 bases of exon-intron or intron-exon boundary sequence with exon sequence in UPPER CASE and intron sequence in lower case.

C. For miRNA inhibiting oligos, provide the miRBase ID of the miRNA and the desired target site (see the miRNA page for details).

Following these instructions will help eliminate mistakes and provide you with a faster return of your design.

We design oligos to avoid motifs that form stable local secondary structures or decrease water solubility. We do not BLAST sequences to check for homology with other published genes. If you find unacceptable homology, we are happy to look for different oligo sequence.