The diagnostics program at Gene Tools is pursuing development of two products: a small-molecule reagent to enhance imaging of tumors and a Morpholino-based diagnostic reagent & instrumentation package for detection of infectious agents.

Contact: Dr. Jon Moulton, Diagnostics and Special Projects

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Here are some references which might be useful to investigators considering using Morpholinos in probe diagnostics systems:

Liu Y, Irving D, Qiao W, Ge D, Levicky R. Kinetic Mechanisms in Morpholino-DNA Surface Hybridization. J. Am. Chem. Soc. 2011;[Epub ahead of print] DOI: 10.1021/ja202631b

Zu Y, Ting AL, Yi G, Gao Z. Sequence-Selective Recognition of Nucleic Acids under Extremely Low Salt Conditions by Using Nanoparticle Probes. Anal Chem. 2011;[Epub ahead of print] DOI: 10.1021/ac2001516

Zu Y, Ting AL, Gao Z. Visualizing Low-Level Point Mutations: Enzyme-like Selectivity Offered by Nanoparticle Probes. Small. 2011 Feb 7;7(3):306-10. doi: 10.1002/smll.201001774. Epub 2010 Dec 22. No abstract available.

Liu G, Dou S, Liu Y, Liang M, Chen L, Cheng D, Greiner D, Rusckowski M, Hnatowich DJ. Unexpected side products in the conjugation of an amine-derivatized morpholino oligomer with p-isothiocyanate benzyl DTPA and their removal. Nucl Med Biol. 2011 Feb;38(2):159-63. Epub 2010 Oct 27.

Li SJ, Li J, Wang K, Wang C, Xu JJ, Chen HY, Xia XH, Huo Q. A Nanochannel Array-Based Electrochemical Device for Quantitative Label-free DNA Analysis. ACS Nano. 2010 Oct 19. [Epub ahead of print]

Cheng D, Wang Y, Liu X, Pretorius PH, Liang M, Rusckowski M, Hnatowich DJ. Comparison of (18)F PET and (99m)Tc SPECT Imaging in Phantoms and in Tumored Mice. Bioconjug Chem. 2010 Aug 18;21(8):1565-70

Tercero N, Wang K, Levicky R. Capacitive Monitoring of Morpholino-DNA Surface Hybridization: Experimental and Theoretical Analysis. Langmuir. 2010 Sep 7;26(17):14351-8.

Gong P, Wang K, Liu Y, Shepard K, Levicky R. Molecular Mechanisms in Morpholino-DNA Surface Hybridization. J Am Chem Soc. 2010 Jun 24. [Epub ahead of print]

Zhang GJ, Luo ZH, Huang MJ, Tay GK, Lim EJ. Morpholino-functionalized silicon nanowire biosensor for sequence-specific label-free detection of DNA. Biosens Bioelectron. 2010 Apr 9. [Epub ahead of print]

Tercero N, Wang K, Gong P, Levicky R. Morpholino Monolayers: Preparation and Label-free DNA Analysis by Surface Hybridization. J Am Chem Soc. 2009 Mar 18. [Epub ahead of print]

Wang X, Smirnov S. Label-Free DNA Sensor Based on Surface Charge Modulated Ionic Conductance. ACS Nano. 2009 Mar 13. [Epub ahead of print]

Gao Z, Boon PT. A DNA biosensor based on a morpholino oligomer coated indium-tin oxide electrode and a cationic redox polymer. Analyst. 2009; [Epub ahead of print]

Wages JM Jr; Wages GM; Matthews P; Weller D; Summerton J. Affinity purification of RNA: sequence-specific capture by nonionic morpholino probes. Biotechniques. 1997 Dec;23(6):1116-21.