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J David Lambert's notes on oligo handling, autoclaving

From the paper:
Lambert JD, Johnson AB, Hudson CN, Chan A. Dpp/BMP2-4 Mediates Signaling from the D-Quadrant Organizer in a Spiralian Embryo. Curr Biol. 2016;[Epub ahead of print] doi:10.1016/j.cub.2016.05.059

From Supplemental Information: Supplemental Experimental Procedures: Injection and knockdown with antisense morpholino oligos:
"We have made some improvements to our previously reported injection protocol [S2,
S4]. First, we find that most MOs we use, including IoDppMO3 and 4, will lose activity
2-3 months after they are resuspended, but can be reactivated by autoclaving.
Similarly, we have stopped heating the morpholinos and fluorescent dye before
injection, because this can hasten evaporation and higher concentrations of either MO
stock or the fluorescent dye can cause the MO to lose activity when injected. Especially
with these improvements, we continue to find MOs to be highly reproducible and
invaluable tools for probing gene function in Ilyanassa."

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