You are here

Morpholinos modifying poly-A tailing

Investigators exploring the use of Morpholino oligos to modify poly-A tailing of mRNAs.

Van Etten JL, Nyquist M, Li Y, Yang R, Ho Y, Johnson RM, Ondigi O, Voytas DF, Henzler C, Dehm SM. Targeting a single alternative polyadenylation site coordinately blocks expression of androgen receptor mRNA splice variants in prostate cancer. Cancer Res. 2017 Aug 15. pii: canres.0320.2017. doi: 10.1158/0008-5472.CAN-17-0320. [Epub ahead of print]

Gong Q, Zhou Z. Regulation of Isoform Expression by Blocking Polyadenylation Signal Sequences with Morpholinos. Methods Mol Biol. 2017;1565:141-150. doi: 10.1007/978-1-4939-6817-6_12.

Mueller AA, van Velthoven CT, Fukumoto KD, Cheung TH, Rando TA. Intronic polyadenylation of PDGFRα in resident stem cells attenuates muscle fibrosis. Nature. 2016 Nov 28. doi: 10.1038/nature20160. [Epub ahead of print]
Vivo-MO in mouse targeting intronic poly-A site, another for a splice site.]

Chen JC, King OD, Zhang Y, Clayton NP, Spencer C, Wentworth BM, Emerson CP Jr, Wagner KR. Morpholino-mediated Knockdown of DUX4 Toward Facioscapulohumeral Muscular Dystrophy Therapeutics. Mol Ther. 2016 Jul 5. doi: 10.1038/mt.2016.111. [Epub ahead of print]

Marsollier AC, Ciszewski L, Mariot V, Popplewell L, Voit T, Dickson G, Dumonceaux J. Antisense targeting of 3'end elements involved in DUX4 mRNA processing is an efficient therapeutic strategy for Facioscapulohumeral Dystrophy: a new gene silencing approach. Hum Mol Genet. 2016 Jan 19. pii: ddw015. [Epub ahead of print]

Makino S, Mishima Y, Inoue K, Inada T. Roles of mRNA fate modulators Dhh1 and Pat1 in TNRC6-dependent gene silencing recapitulated in yeast. J Biol Chem. 2015 Mar 27;290(13):8331-47. doi: 10.1074/jbc.M114.615088. Epub 2015 Feb 5.

Gong Q, Stump MR, Zhou Z. Upregulation of Functional Kv11.1 Isoform Expression by Inhibition of Intronic Polyadenylation with Antisense Morpholino Oligonucleotides. J Mol Cell Cardiol. 2014 Nov;76:26-32. doi: 10.1016/j.yjmcc.2014.08.007. Epub 2014 Aug 14.

Uehara H, Cho Y, Simonis J, Cahoon J, Archer B, Luo L, Das SK, Singh N, Ambati J, Ambati BK. Dual suppression of hemangiogenesis and lymphangiogenesis by splice-shifting morpholinos targeting vascular endothelial growth factor receptor 2 (KDR). FASEB J, 2012;[Epub ahead of print] doi:10.1096/fj.12-213835

Wada T, Hara M, Taneda T, Qingfu C, Takata R, Moro K, Takeda K, Kishimoto T, Handa H. Antisense morpholino targeting just upstream from a poly(A) tail junction of maternal mRNA removes the tail and inhibits translation. Nucl Acids Res. 2012;[Epub ahead of print] doi:10.1093/nar/gks765

Vorlová S, Rocco G, LeFave CV, Jodelka FM, Hess K, Hastings ML, Henke H, Cartegni L. Induction of Antagonistic Soluble Decoy Receptor Tyrosine Kinases by Intronic PolyA Activation. Mol Cell. 2011;(43)6:927-39.