You are here

Jon's Blog

Post date
Genetic compensation: A phenomenon in search of mechanisms

Genetic compensation: A phenomenon in search of mechanisms.

El-Brolosy MA, Stainier DYR.

PLoS Genet. 2017 Jul 13;13(7):e1006780. doi: 10.1371/journal.pgen.1006780. eCollection 2017 Jul. Review.

http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.10...

Friday, July 14, 2017 - 10:29
Book: Morpholino Oligomers

Hong and I edited a book: Morpholino Oligomers. 2017. Methods in Molecular Biology. Volume 1565. Humana Press (Springer). doi:10.1007/978-1-4939-6817-6

Links to chapters follow.

1. Summerton JE. Invention and Early History of Morpholinos: From Pipe Dream to Practical Products. Methods Mol Biol. 2017;1565:1-15. doi: 10.1007/978-1-4939-6817-6_1. Abstract: PubMed

Tuesday, April 11, 2017 - 01:57
Review of FDA-approved antisense (2017)

FDA-Approved Oligonucleotide Therapies in 2017.
Stein CA, Castanotto D.
Mol Ther. 2017 Mar 30. pii: S1525-0016(17)30122-3. doi: 10.1016/j.ymthe.2017.03.023. [Epub ahead of print] Review.
http://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(17)30122-3

Thursday, April 6, 2017 - 08:40
Using Morpholinos to Control Gene Expression (in CPNAC)

Wiley has published an update to my Morpholino how-to and background review.

Moulton JD. Using Morpholinos to Control Gene Expression. Curr Protoc Nucleic Acid Chem. 2017 Mar 2;68:4.30.1-4.30.29. doi: 10.1002/cpnc.21.

http://onlinelibrary.wiley.com/doi/10.1002/cpnc.21/abstract

Saturday, March 4, 2017 - 03:26
Fructose and uptake of Morpholinos

This is about fructose and Morpholinos.

http://oregonstate.edu/ua/ncs/archives/2017/mar/often-villain-fructose-m...

Here is the original paper being discussed.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5022132/

Friday, March 3, 2017 - 08:58
nasal administration of delivery-enabled Morpholino in mice

This paper is an example of nasal administration of a delivery-enabled Morpholino in mice.

Soonthornvacharin S, Rodriguez-Frandsen A, Zhou Y, Galvez F, Huffmaster NJ, Tripathi S, Balasubramaniam VRMT, Inoue A, de Castro E, Moulton H, Stein DA, Sánchez-Aparicio MT, De Jesus PD, Nguyen Q, König R, Krogan NJ, García-Sastre A, Yoh SM, Chanda SK. Systems-based analysis of RIG-I-dependent signalling identifies KHSRP as an inhibitor of RIG-I receptor activation. Nat Microbio. 2017;2:17022. doi:10.1038/nmicrobiol.2017.22

Friday, March 3, 2017 - 06:55
Review: Eteplirsen in the treatment of Duchenne muscular dystrophy

Lim KRQ, Maruyama R, Yokota T. Eteplirsen in the treatment of Duchenne muscular dystrophy. Drug Design, Dev & Ther. 2017;11:533-45. doi:DDDT.S97635

Open access

https://www.dovepress.com/eteplirsen-in-the-treatment-of-duchenne-muscul...

Wednesday, March 1, 2017 - 08:06
Abdominal injection of Vivo-Morpholino in fish for oocyte knockdown

Injection of Vivo-Morpholinos into the abdominal cavity of medaka was used to knock down mPRα in the oocytes.

Roy SR, Wang J, Rana MR, Nakashima M, Tokumoto T. Characterization of membrane progestin receptor α (mPRα) of the medaka and role in the induction of oocyte maturation. Biomed Res. 2017;38(1):79-87. doi: 10.2220/biomedres.38.79.

https://www.jstage.jst.go.jp/article/biomedres/38/1/38_79/_article

Wednesday, March 1, 2017 - 04:04
TEDx Perth talk on Morpholinos and Duchenne muscular dystrophy (Wilton, late 2016)

Prof. Steve Wilton: TEDx Perth talk on Morpholinos and Duchenne muscular dystrophy.

If you have had time with Steve, life is better. He's a good sort.

Thursday, February 16, 2017 - 01:12
Which splice junction to target, predicting cryptic splice sites, and doing the two-non-overlapping oligo specificity experiment

I've had the pleasure of corresponding with Yuhong Liang (梁雨虹)of 4A Biotech in China; she is the product manager for Morpholino oligos at 4A Biotech. We have been working with 4A for a little over a year now and Yuhong has been learning very quickly, but is still new enough to the Morpholino field that she is asking questions of broad interest. She had some questions regarding which splice junction to target, predicting cryptic splice sites, and the process of doing the two-non-overlapping oligo specificity experiment.

Wednesday, February 8, 2017 - 01:07

Pages