Wiley-Liss Inc. publishers has posted the entire Genesis Morpholino special issue on the web.

Jon Moulton is posting notes on some Morpholino papers in his blog:
http://blog.myspace.com/127542408

A Morpholino discussion group on Nature Networks has been established.

Sections below:

• Controls & calibration
• Zebrafish
• Xenopus
• Chick
• Databases

Review of Morpholino controls, recommended with enthusiasm!
Eisen JS, Smith JC. Controlling morpholino experiments: don't stop making antisense. Development. 2008 May;135(10):1735-43. Epub 2008 Apr 9.

See the supporting online material of the following citation for methods for characterizing microinjected volume.
Riedel-Kruse IH, Muller C, Oates AC. Synchrony dynamics during initiation, failure, and rescue of the segmentation clock. Science. 2007 Sep 28;317(5846):1911-5. Epub 2007 Aug 16.

USA Commercial Drug Discovery Customers!
Are you interested in using Morpholinos in zebrafish, or in having another firm do your Morpholino injections for you? Zygogen offers flexible research and drug discovery licenses as well as contract research services. For-Profit Entities in the USA wishing to use Morpholinos in zebrafish are required to obtain a license from Zygogen (www.zygogen.com). For license or contract research information, please call: 404-523-7309 or email: info@zygogen.com.

A database of zebrafish Morpholinos is available at Zfin, the Zebrafish Information Network. Look for Morpholino in the pulldown menu.

The New England Zebrafish Husbandry Association (NEZHA) is a professional organization dedicated to promoting the improvement of husbandry standards for usage of the zebrafish (Danio rerio) in scientific research.

This miRNA protocol by Wigard Kloosterman describes using Morpholinos to knockdown miRNA and LNA to detect miRNA in the zebrafish.

Cerda GA, Thomas JE, Allende ML, Karlstrom RO, Palma V. Electroporation of DNA, RNA, and morpholinos into zebrafish embryos. Methods. 2006 Jul;39(3):207-11.

Mary Mullins of U. Penn. has posted at MBL a zebrafish microinjection through the chorion protocol for a lab course along with an intro for the Morpholino lab.

Paper on electroporation into the embryonic Xenopus CNS:
Falk J, Drinjakovic J, Leung KM, Dwivedy A, Regan AG, Piper M, Holt CE. Electroporation of cDNA/Morpholinos to targeted areas of embryonic CNS in Xenopus. BMC Developmental Biology 2007, 7:107 [epub ahead of print]

Abstract of a paper describing an electroporation protocol optimized for Xenopus tadpole brains:
Bestman JE, Ewald RC, Chiu SL, Cline HT. In vivo single-cell electroporation for transfer of DNA and macromolecules. Nat Protoc. 2006;1(3):1267-72.

The Smith lab at the Gurdon Institute has posted results of their Morpholino screen in Xenopus tropicalis.

The Harland lab at Berkeley has posted a protocol for microinjecting Morpholinos into Xenopus tropicalis oocytes.

Carol Erickson's lab at UC Davis posted this chick egg electroporation protocol.

Voiculescu O, Papanayotou C, Stern CD. Spatially and temporally controlled electroporation of early chick embryos. Nat Protoc. 2008;3(3):419-26.

NCBI Entrez Browser for access to GenBank, Medline files and other biotech resources.

The Ensembl Genome Browser for good markups of introns and exons in transcripts, useful for splice blocking Morpholino design.

The Sanger Institute's miRBase for microRNA sequence and structure information.