Methods and Protocols

Live Help

Text chat with Gene Tools Customer Support biologists.

Online Store Login

Enter our online store to order Gene Tools products.

Navigation

Publications Search

Site Search

Zebrafish contract research and commercial licenses

For contract research using Morpholinos in zebrafish or to license Morpholinos for commercial use in zebrafish in the USA, contact Zygogen, Inc. See the Outside Resources box for more information.

Delivery into Embryos

The journal genesis: the Journal of Genetics and Development devoted an entire issue to the use of Morpholino oligos. The nice folks at Wiley have made this entire journal issue open-access. In this issue you will find delivery methods and results for zebrafish, Xenopus, chick, and mouse embryos, and you will also find the first use of splice-blocking (exon deletion) in a developmental system.

Complete genesis issue, July 2001
PDF - Draper paper describing splice-blocking in zebrafish

Delivery into Mammalian Cells

Endo-Porter delivery
Scrape delivery
Special delivery: no longer available from Gene Tools

protocol for assessing delivery into cultured cells

Delivery into Adult Organisms

Vivo-Morpholinos

Quantitating your Morpholino oligo

Here is our method for determining the concentration of your Morpholino oligo that is dissolved in water.

PDF - Quantitating Morpholino oligo concentration

Other protocols

Additional protocols can be found in the Customer Support: Downloads section of our website.