Methods and Protocols

Live Help

Text chat with Gene Tools Customer Support biologists.

Online Store Login

Enter our online store to order Gene Tools products.

Navigation

Publications Search

Site Search

Zebrafish contract research and commercial licenses

For contract research using Morpholinos in zebrafish or to license Morpholinos for commercial use in zebrafish in the USA, contact Zygogen, Inc. See the Outside Resources box for more information.

Delivery into Mammalian Cells

Scrape delivery
Special delivery (EPEI)
PDF - EPEI delivery paper describing the mechanism

Delivery into Embryos

The Genesis journal devoted an entire issue to the use of Morpholino oligos. The nice folks at Wiley have made this entire journal issue available to the public. In this issue you will find delivery methods and results for zebrafish, xenopus, chick, and Drosophila.

You will also find the first use of splice-blocking (exon deletion) in a developmental system.

Complete genesis issue, July 2001
PDF - Draper paper describing splice-blocking in zebrafish

Quantitating your Morpholino oligo

Here is our method for determining the concentration of your Morpholino oligo that is dissolved in water.

PDF - Quantitating Morpholino oligo concentration

Other protocols

Additional protocols can be found in the Customer Support: Downloads section of our website.