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Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate

Authors: 
Araya C, Häkkinen H-M, Carcamo L, Cerda M, Savy T, Rookyard C, Peyriéras N, Clarke JDW
Citation: 
Sci Rep. 2019;9:1835. doi:10.1038/s41598-018-38455-w
Abstract: 
Tissue internalisation is a key morphogenetic mechanism by which embryonic tissues generate complex internal organs and a number of studies of epithelia have outlined a general view of tissue internalisation. Here we have used quantitative live imaging and mutant analysis to determine whether similar mechanisms are responsible for internalisation in a tissue that apparently does not have a typical epithelial organisation – the zebrafish neural plate. We found that although zebrafish embryos begin neurulation without a conventional epithelium, medially located neural plate cells adopt strategies typical of epithelia in order to constrict their dorsal surface membrane during cell internalisation. Furthermore, we show that Myosin-II activity is a significant driver of this transient cell remodeling which also depends on Cdh2 (N-cadherin). Abrogation of Cdh2 results in defective Myosin-II distribution, mislocalised internalisation events and defective neural plate morphogenesis. Our work suggests Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate.
Organism or Cell Type: 
zebrafish
Delivery Method: 
microinjection