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Jon's Blog

		Post date
(Methods Cell Biol):Tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish	Cardenas-Rodriguez M, Drummond IA. The challenge of dissecting gene function in model organisms: Tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish. Methods Cell Biol. 2023;176:1-25. doi: 10.1016/bs.mcb.2022.12.019. Epub 2023 Jan 27. https://www.sciencedirect.com/science/article/abs/pii/S0091679X22002072	Thursday, May 11, 2023 - 15:29
I have an article in Pharma's Almanac - Morpholino stories	Morpholino article in Pharma's Almanac	Friday, May 5, 2023 - 15:34
(Review) Recent Trends in Antisense Therapies for Duchenne Muscular Dystrophy	Recent Trends in Antisense Therapies for Duchenne Muscular Dystrophy. Wilton-Clark H, Yokota T. Pharmaceutics. 2023; 15(3):778. https://doi.org/10.3390/pharmaceutics15030778 https://www.mdpi.com/1999-4923/15/3/778	Thursday, March 30, 2023 - 14:23
Morpholinos shorter than 25 bases	A Morpholino user, accustomed to using 25-base Morpholinos in Xenopus laevis, was concerned about a design she received for a 20-base Morpholino. She wrote to ask if we had designed many short oligos and if they worked well. Here is my response. -------------------------------------	Wednesday, February 15, 2023 - 11:13
Molecular dynamics of aqueous Morpholino oligos	Maksudov F, Kliuchnikov E, Pierson D, Ujwal ML, Marx KA, Chanda A, Barsegov V. Therapeutic Phosphorodiamidate Morpholino Oligonucleotides, an RNA mimic: Physical Properties, Solution Structures, and Folding Thermodynamics. Mol Ther Nucleic Acids. 2023;[Epub] doi:10.1016/j.omtn.2023.02.007 https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S21...(23)00027-6	Wednesday, February 15, 2023 - 10:15
Discussion of RNA change on adding translation blocking Morpholino	From: **** Sent: Wednesday, January 18, 2023 10:17 AM To: Jon Moulton Subject: Translation-blocking Vivo-MO reduces gene expression on the level of mRNA? Hello Jon,	Wednesday, January 18, 2023 - 12:35
History: exon skipping	Lim, K.R.Q., Yokota, T. (2018). Invention and Early History of Exon Skipping and Splice Modulation. In: Yokota, T., Maruyama, R. (eds) Exon Skipping and Inclusion Therapies. Methods in Molecular Biology, vol 1828. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8651-4_1 https://link.springer.com/protocol/10.1007/978-1-4939-8651-4_1	Tuesday, January 17, 2023 - 11:03
Master’s thesis based on microarray analysis of WT, Morphant and rescue fish	Master’s thesis based on microarray analysis of WT, Morphant and rescue fish Tuttle, Matthew Alan (2017) In silico analysis of zebrafish leptin-a knockdown gene expression data reveals enrichment for metabolic and developmental pathways including morpholino artifacts https://etd.ohiolink.edu/apexprod/rws_olink/r/1501/10?clear=10&p10_acces...	Monday, January 16, 2023 - 11:32
Vivo-Morpholino uptake by zebrafish larvae at 4dpf	Liang H, Li Y, Li M, Zhou W, Chen J, Zhang Z, Yang Y, Ran C, Zhou Z. The effect and underlying mechanism of yeast β-glucan on antiviral resistance of zebrafish against spring viremia of carp virus infection. Front Immunol. 2022 Nov 3;13:1031962. doi: 10.3389/fimmu.2022.1031962. eCollection 2022. https://www.frontiersin.org/articles/10.3389/fimmu.2022.1031962/full	Monday, November 21, 2022 - 09:48
Vivo-Morpholinos as switching molecules for virally-delivered targets in mice	The authors of this new paper use an engineered viral vector (AAV) to express a secreted antibody. They make a version where the antibody is out-of-frame and a Morpholino is used to skip an exon, bring the antibody in-frame and switch on its functional expression; the target is a payload of the AAV and the switching molecule is the Morpholino. This use of Morpholinos as switching molecules in engineered systems has great therapeutic potential. In the same paper, they controlled the expression of their AAV cargo with a Vivo-Morpholino after delivery into a mouse.	Wednesday, August 10, 2022 - 12:14

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