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Jon's Blog
| Post date | ||
|---|---|---|
| Example of specificity control using a Morpholino in its mutant |
This paper is a nice example of Morpholino specificity control by using a Morpholino in its corresponding mutant. Chambers BE, Gerlach GF, Clark EG, Chen KH, Levesque AE, Leshchiner I, Goessling W, Wingert RA. Tfap2a is a novel gatekeeper of nephron differentiation during kidney development. Development. 2019 Jun 3. pii: dev.172387. doi: 10.1242/dev.172387. [Epub ahead of print] |
Wednesday, July 10, 2019 - 11:53 |
| Targeting mitochondrially-encoded circular RNAs in the cytosol: mitochondrial protein uptake suppression |
Here is an interesting report of Morpholinos targeting mitochondrially-encoded circular RNAs in the cytosol to prevent RNA-chaperoned protein uptake into the mitochondria. Liu X, Wang X, Li J, Hu S, Deng Y, Yin H, Bao X, Zhang QC, Wang G, Wang B, Shi Q, Shan G. The identification of mecciRNAs and their roles in mitochondrial entry of proteins. bioRxiv. 2019;[preprint] doi:10.1101/668665 |
Friday, July 5, 2019 - 12:32 |
| Dosing mama mouse i.v. for transplacental delivery of Vivo-Morpholinos to pups |
Touma M, Kang X, Gao F, Zhao Y, Cass AA, Biniwale R, Xiao X, Eghbali M, Coppola G, Reemtsen B, Wang Y. Wnt11 regulates cardiac chamber development and disease during perinatal maturation. JCI Insight. 2017 Sep 7;2(17). pii: 94904. doi: 10.1172/jci.insight.94904. eCollection 2017 Sep 7. |
Friday, May 31, 2019 - 12:00 |
| Review - Antisense-mediated splice intervention to treat human disease: the odyssey continues |
Pitout I, Flynn LL, Wilton SD, Fletcher S. Antisense-mediated splice intervention to treat human disease: the odyssey continues. F1000Research 2019,8(F1000 Faculty Rev):710 doi:10.12688/f1000research.18466.1 |
Wednesday, May 22, 2019 - 13:51 |
| Innate immune stimulation by Morpholinos in Xenopus? |
The discussion of innate immune stimulation by Morpholinos in Xenopus continues. Paraiso KD, Blitz IL, Zhou JJ, Cho KWY. Morpholinos Do Not Elicit an Innate Immune Response during Early Xenopus Embryogenesis. Dev Cell. 2019;49(4):643-50. doi:10.1016/j.devcel.2019.04.019 |
Monday, May 20, 2019 - 14:37 |
| Their mutants don't show a phenotype and their morphants don't: they suggest that more time is needed for compensation |
These investigators suggest a reason their mutants don't show a phenotype and their morphants don't. The mutant embryos have had time enough to compensate for the knockdowns, hiding the phenotype, while the acute knockdown of the Morpholino shows a phenotype during time insufficient for compensation. Yang C-R, Rajkovic G, Daldello EM, Luong XG, Chen J, Conti M. The RNA binding protein DAZL functions as repressor and activator of maternal mRNA translation during oocyte maturation. bioRxiv. 2019;[preprint] doi:10.1101/598805 |
Wednesday, May 15, 2019 - 10:28 |
| Antisense oligonucleotides to treat spinal muscular atrophy (Shift Pharmaceuticals) |
Antisense oligonucleotides to treat spinal muscular atrophy |
Monday, May 6, 2019 - 10:13 |
| Morpholinos: Research Tools and Therapeutics (Cell Press Selections) |
New Cell Press Selections: Morpholinos: Research Tools and Therapeutics |
Friday, April 26, 2019 - 14:07 |
| Storage of Morpholinos: refrigerate or room temperature? |
Morpholino oligos are completely chemically & biologically (enzymatically) stable sitting in water at 4C or at room temperature as long as the pH is not extreme. However, they undergo physical changes that can affect their biological activity. Chilled storage increases the tendency of the oligos to associate with container walls (this is especially a problem if the oligos undergo freeze-thaw cycles). Once a Morpholino has associated tightly with the wall of a container, it can be difficult or impossible to get the oligo back into solution at neutral pH. |
Tuesday, April 23, 2019 - 08:01 |
| Proposed mechanism underlying genetic compensation |
Genetic paradox explained by nonsense. Wilkinson MF. Nature. 2019;568:179-180. doi: 10.1038/d41586-019-00823-5 Genetic compensation triggered by mutant mRNA degradation. El-Brolosy MA et al. Nature. 2019;568:193-197. doi:10.1038/s41586-019-1064-z |
Thursday, April 11, 2019 - 13:46 |
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