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Water for Morpholino stocks - distilled? Milli-Q? DEPC?

It is important to use RNase-free water for dissolution of RNA, but not Morpholinos Trace diethylpyrocarbonate (DEPC) left in the water can damage Morpholinos by alkylating bases, but the DEPC can be degraded by autoclaving. Morpholinos are not degraded by RNases, even if the enzyme and oligo are incubated together under favorable conditions for the reaction. Morpholinos have been incubated with rat liver lysates and extracted from animal tissue after in vivo treatment, all without detecting degradation of the Morpholino.

Friday, February 16, 2024 - 09:53
E1v1.11: a Morpholino antisense oligo for Spinal Muscular Atrophy

Optimization and Safety Results of E1v1.11: a morpholino antisense oligonucleotide therapeutic for Spinal Muscular Atrophy.

Oconnor S, Hansen H, Osman E, Kaifer KD, Morcos P, Kacher M, Beatty D, Lorson C.

MDA Conference Abstracts. 2024;Poster 85

Tuesday, November 14, 2023 - 13:29
Video interview: Jon on splice modification with Morpholinos

Here's an interview with Jon Moulton on Pharma's Almanac discussing using Morpholinos to alter splicing.

Tuesday, November 14, 2023 - 10:41
E1i1 oligo activates cryptic site in exon 1

Here's a report of an unusual splice response to a Morpholino targeting the e1i1 junction. We'd expect the common outcome, insertion of intron 1. Instead, the splice was redirected to a cryptic splice site in exon 1, resulting in a shortened mature transcript. The new splice site knocked the downstream sequence out of frame, so this produced a good knockdown.

Monday, November 13, 2023 - 11:00
in silico Morpholino sequence design

Here's another computer-based oligo sequence picker.

Pacelli C, Rossi A, Milella M, Colomb T, Le Pera L. RNA-Based Strategies for Cancer Therapy: In Silico Design and Evaluation of ASOs for Targeted Exon Skipping. Int. J. Mol. Sci. 2023, 24, 14862. 10.3390/ijms241914862

Wednesday, October 11, 2023 - 12:41
Delivery of Morpholinos by irradiation

Morpholino oligonucleotides useful in cancer treatment
Includes delivery of Morpholinos by irradiation

Thursday, July 13, 2023 - 15:33
(Methods Cell Biol):Tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish

Cardenas-Rodriguez M, Drummond IA. The challenge of dissecting gene function in model organisms: Tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish. Methods Cell Biol. 2023;176:1-25. doi: 10.1016/bs.mcb.2022.12.019. Epub 2023 Jan 27.

Thursday, May 11, 2023 - 15:29
I have an article in Pharma's Almanac - Morpholino stories

Morpholino article in Pharma's Almanac

Friday, May 5, 2023 - 15:34
(Review) Recent Trends in Antisense Therapies for Duchenne Muscular Dystrophy

Recent Trends in Antisense Therapies for Duchenne Muscular Dystrophy.
Wilton-Clark H, Yokota T. Pharmaceutics. 2023; 15(3):778.

Thursday, March 30, 2023 - 14:23
Morpholinos shorter than 25 bases

A Morpholino user, accustomed to using 25-base Morpholinos in Xenopus laevis, was concerned about a design she received for a 20-base Morpholino. She wrote to ask if we had designed many short oligos and if they worked well. Here is my response.


Wednesday, February 15, 2023 - 11:13