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Jon's Blog
| Post date | ||
|---|---|---|
| Paper: Induction of cryptic pre-mRNA splice-switching by antisense oligonucleotide |
Mostly phosphorothioate work Ham KA, Keegan NP, McIntosh CS, Aung-Htut MT, Zaw K, Greer K, Fletcher Sue, Wilton SD. Induction of cryptic pre-mRNA splice-switching by antisense oligonucleotides. Sci Rep. 2021;11(1):15137 doi:10.1038/s41598-021-94639-x |
Friday, July 23, 2021 - 14:22 |
| Genetic compensation in soybean (no Morpholino) |
Genetic compensation in soybean: I am putting this in the blog as an example of an organism distant from zebrafish which is also compensating for mutation. |
Friday, July 16, 2021 - 14:42 |
| Nice introduction to splice-modification therapy from OTS |
From the Oligonucleotide Therapeutics Society: "Can Oligonucleotides Target RNA Splicing to Treat Disease?" |
Wednesday, June 23, 2021 - 09:41 |
| Mutant-morphant mismatch but no genetic compensation |
Zebrafish Chromosome 14 Gene Differential Expression in the fmr1 h u2787 Model of Fragile X Syndrome. https://www.frontiersin.org/articles/10.3389/fgene.2021.625466/full |
Friday, June 18, 2021 - 08:14 |
| Morphant vs mutant: "...this knockout line is deemed unsuitable for our study." |
Here’s a nice description of why, in this study, morphants are preferable to mutants. |
Friday, June 11, 2021 - 11:53 |
| Review of neurodegenerative disease and splice modulation |
Neurodegenerative diseases: a hotbed for splicing defects and the potential therapies. https://translationalneurodegeneration.biomedcentral.com/articles/10.118... |
Monday, May 24, 2021 - 13:32 |
| Therapeutic Modulation of RNA Splicing in Malignant and Non-Malignant Disease (Review) |
Therapeutic Modulation of RNA Splicing in Malignant and Non-Malignant Disease. |
Monday, May 17, 2021 - 11:33 |
| SARS-CoV-2 RNA targets for steric blocking |
Potantial targets for steric blocking on the SARS-CoV-2 genome and transcripts. The SARS-CoV-2 RNA interactome. https://www.sciencedirect.com/science/article/pii/S1097276521003270 |
Monday, May 17, 2021 - 10:23 |
| Positive control, human and mouse splice modification |
Flynn LL, Mitrpant C, Adams A, Pitout IL, Stirnweiss A, Fletcher S, Wilton SD. Targeted SMN Exon Skipping: A Useful Control to Assess In Vitro and In Vivo Splice-Switching Studies. Biomedicines. 2021;9(5):552. doi:10.3390/biomedicines9050552 |
Friday, May 14, 2021 - 11:52 |
| MO knockdown of ectopic GFP in axlotl |
There are few papers reporting using a Morpholino to directly target GFP in vivo; here is one example. |
Friday, April 23, 2021 - 11:21 |
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