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Videos involving Gene Tools products on JoVE

The Journal of Visualized Experiments sometimes publishes video articles involving Morpholino oligos. Here are links to some of these videos.

Open Access Articles

JoVE Science Education Database. Developmental Biology. Gene Silencing with Morpholinos. JoVE, Cambridge, MA, (2022).

de Calbiac H, Dabacan A, Muresan R, Kabashi E, Ciura S. Behavioral And Physiological Analysis In A Zebrafish Model Of Epilepsy. J Vis Exp. 2021 Oct 19;(176). doi: 10.3791/58837.

Nordin JZ, Mizobe Y, Nakamura H, Komaki H, Takeda S, Aoki Y. Characterizing Exon Skipping Efficiency in DMD Patient Samples in Clinical Trials of Antisense Oligonucleotides. J Vis Exp. 2020 May 7;(159). doi: 10.3791/60672.
Analyzing samples from patients treated with NS Pharma's Morpholino drug, viltolarsen.

Welch EL, Eno CC, Nair S, Lindeman RE, Pelegri F. Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish. JoVE. 2017;122:e55213. doi:10.3791/55213

Kari W, Zeng F, Zitzelsberger L, Will J, Rothbächer U. Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis. J Vis Exp. 2016 Oct 16;(116). doi: 10.3791/54313.

Miskew Nichols B, Aoki Y, Kuraoka M, Lee JJ, Takeda S, Yokota T. Multi-exon Skipping Using Cocktail Antisense Oligonucleotides in the Canine X-linked Muscular Dystrophy. J Vis Exp. 2016 May 24;(111). doi: 10.3791/53776.

Perner B, Schnerwitzki D, Graf M, Englert C. Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning. J Vis Exp. 2016;110:e53921. doi:10.3791/53921

Mazon Moya MJ, Colucci-Guyon E, Mostowy S. Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions. JoVE. 2014;(91):e51601. doi:10.3791/51601

Niederriter, A. R., Davis, E. E., Golzio, C., Oh, E. C., Tsai, I. C., Katsanis, N. In Vivo Modeling of the Morbid Human Genome using Danio rerio. J Vis Exp. 2013;78:e50338. doi:10.3791/50338.

Kizil C, Iltzsche A, Kaslin J, Brand M. Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides. J Vis Exp. 2013;(75):e50415, doi:10.3791/50415

Hyde, D. R., Godwin, A. R., Thummel, R. In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin. J. Vis. Exp. (61), e3632, doi:10.3791/3632 (2012).

Loucks, E., Ahlgren, S. Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure. J. Vis. Exp. (61), e3704, doi:10.3791/3704 (2012).

Holmes, K. E., Wyatt, M. J., Shen, Y., Thompson, D. A., Barald, K. F. Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development. J. Vis. Exp. (47), e2466, doi:10.3791/2466 (2011).

Thummel, R., Bailey, T. J., Hyde, D. R. In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina. J. Vis. Exp. (58), e3603, doi:10.3791/3603 (2011).

Tabler, J. M., Liu, K. J. Electroporation of Craniofacial Mesenchyme. J. Vis. Exp. (57), e3381, doi:10.3791/3381 (2011).

Rosen, J. N., Sweeney, M. F., Mably, J. D. Microinjection of Zebrafish Embryos to Analyze Gene Function. J. Vis. Exp. (25), e1115, doi:10.3791/1115 (2009).

Subscription Access Articles

Zakaria ZZ, Eisa-Beygi S, Benslimane FM, Ramchandran R, Yalcin HC. Design and Microinjection of Morpholino Antisense Oligonucleotides and mRNA into Zebrafish Embryos to Elucidate Specific Gene Function in Heart Development. J Vis Exp. 2022 Aug 9;(186). doi: 10.3791/63324.

Sharma B, Subramaniam YJ, Ayyappa Raja D, Aggarwal A, Sivasubbu S, Natarajan VT. Reverse Genetic Approach to Identify Regulators of Pigmentation using Zebrafish. J Vis Exp. 2022 Mar 1;(181):e62955. doi: 10.3791/62955.

Cafora M, Forti F, Briani F, Ghisotti D, Pistocchi A. Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos. J Vis Exp. 2020;(159):e61275. doi:10.3791/61275

Wang Y, Zhang D, Zhou F, Zhou M, Li Q, Chen J, Yang J. Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development. J Vis Exp. 2020 May 28;(159). doi: 10.3791/60498.

de Calbiac H, Dabacan A, Muresan R, Kabashi E, Ciura S. Behavioral And Physiological Analysis In A Zebrafish Model Of Epilepsy. J Vis Exp. 2020:e58837. doi:10.3791/58837

Stahl BA, Jaggard JB, Chin JS, Kowalko JE, Keene AC, Duboué ER. Manipulation of Gene Function in Mexican Cavefish. J Vis Exp. 2019;146:e59093, doi:10.3791/59093.

Ohk J, Jung H. Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis. J Vis Exp. 2017;(123):e55652. doi:10.3791/55652

Dudczig S, Currie PD, Poggi L, Jusuf PR. In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences>. J Vis Exp. 2017 Mar 22;(121). doi: 10.3791/55490.

JoVE Science Education Database. Essentials of Developmental Biology. Gene Silencing with Morpholinos. JoVE. 2017. doi:10.3791/5326

Range RC, Martinez-Bartolomé M, Burr SD. The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions. J Vis Exp. 2017;120:e55113. doi:10.3791/55113

Hagedorn EJ, Cillis JL, Curley CR, Patch TC, Li B, Blaser BW, Riquelme R, Zon LI, Shah DI. Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae. J Vis Exp. 2016;(112),e54168, doi:10.3791/54168

Molina-Villa T, Mendoza V, López-Casillas F. Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos. J Vis Exp. 2016 Aug 31;(114). doi: 10.3791/54405

Giger FA, Dumortier JG, David NB. Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos. J Vis Exp. 2016;110):e53792. doi:10.3791/53792

Bernut A, Dupont C, Sahuquet A, Herrmann JL, Lutfalla G, Kremer, L. Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos. J Vis Exp. 2015;(103),e53130, doi:10.3791/53130

Christou-Savina S, Beales PL, Osborn DPS. Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay. J Vis Exp. 2015;96:e52540. doi:10.3791/52540

Jacox LA, Dickinson AJ, Sive H. Facial Transplants in Xenopus laevis Embryos. J Vis Exp. 2014;(85):e50697, doi:10.3791/50697

Huang L, Xiao A, Wecker A, McBride DA, Choi SY, Zhou W, Lipschutz JH. A Possible Zebrafish Model of Polycystic Kidney Disease: Knockdown of wnt5a Causes Cysts in Zebrafish Kidneys. J Vis Exp. 2014;94:e52156. doi:10.3791/52156.

Cheatle Jarvela AM, Hinman V. A Method for Microinjection of Patiria minata Zygotes. J Vis Exp. 2014 Sep 1;(91). doi: 10.3791/51913.

Mugoni V, Camporeale A, Santoro MM. Analysis of Oxidative Stress in Zebrafish Embryos. J Vis Exp. 2014;(89),e51328. doi:10.3791/51328.

Ljubimova JY, Ding H, Portilla-Arias J, Patil R, Gangalum PR, Chesnokova A, Inoue S, Rekechenetskiy A, Nassoura T, Black KL, Holler E. Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine? J Vis Exp. 2014;(88):e50668. doi:10.3791/50668

Konantz J, Antos CL. Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva. J Vis Exp. 2014;88:e51595 doi:10.3791/51595

Baraban SC. Forebrain Electrophysiological Recording in Larval Zebrafish. J Vis Exp. 2013;(71):e50104. doi:10.3791/50104

Gfrerer L, Dougherty M, Liao EC. Visualization of Craniofacial Development in the sox10: kaede Transgenic Zebrafish Line Using Time-lapse Confocal Microscopy. J Vis Exp. 2013;(79):e50525, doi:10.3791/50525

Smith LL, Beggs AH, Gupta VA. Analysis of skeletal muscle defects in larval zebrafish by birefringence and touch-evoke escape response assays. J Vis Exp. 2013 Dec 13;(82):e50925. doi: 10.3791/50925.

Wang, G., Yost, H. J., Amack, J. D. Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle. J. Vis. Exp. (73), e50038, doi:10.3791/50038 (2013).

Brothers, K. M., Wheeler, R. T. Non-invasive Imaging of Disseminated Candidiasis in Zebrafish Larvae. J. Vis. Exp. (65), e4051, doi:10.3791/4051 (2012).

Rikin, A., Rosenfeld, G. E., McCartin, K., Evans, T. A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis. J. Vis. Exp. (42), e2020, doi:10.3791/2020 (2010).

Yuan, S., Sun, Z. Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos. J. Vis. Exp. (27), e1113, doi:10.3791/1113 (2009).