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Targeting mitochondrially-encoded circular RNAs in the cytosol: mitochondrial protein uptake suppression

Here is an interesting report of Morpholinos targeting mitochondrially-encoded circular RNAs in the cytosol to prevent RNA-chaperoned protein uptake into the mitochondria.

Liu X, Wang X, Li J, Hu S, Deng Y, Yin H, Bao X, Zhang QC, Wang G, Wang B, Shi Q, Shan G. The identification of mecciRNAs and their roles in mitochondrial entry of proteins. bioRxiv. 2019;[preprint] doi:10.1101/668665

Friday, July 5, 2019 - 12:32
Dosing mama mouse i.v. for transplacental delivery of Vivo-Morpholinos to pups

Touma M, Kang X, Gao F, Zhao Y, Cass AA, Biniwale R, Xiao X, Eghbali M, Coppola G, Reemtsen B, Wang Y. Wnt11 regulates cardiac chamber development and disease during perinatal maturation. JCI Insight. 2017 Sep 7;2(17). pii: 94904. doi: 10.1172/jci.insight.94904. eCollection 2017 Sep 7.

Friday, May 31, 2019 - 12:00
Review - Antisense-mediated splice intervention to treat human disease: the odyssey continues

Pitout I, Flynn LL, Wilton SD, Fletcher S.

Antisense-mediated splice intervention to treat human disease: the odyssey continues.

F1000Research 2019,8(F1000 Faculty Rev):710 doi:10.12688/f1000research.18466.1

Wednesday, May 22, 2019 - 13:51
Innate immune stimulation by Morpholinos in Xenopus?

The discussion of innate immune stimulation by Morpholinos in Xenopus continues.

Paraiso KD, Blitz IL, Zhou JJ, Cho KWY. Morpholinos Do Not Elicit an Innate Immune Response during Early Xenopus Embryogenesis. Dev Cell. 2019;49(4):643-50. doi:10.1016/j.devcel.2019.04.019

Monday, May 20, 2019 - 14:37
Their mutants and their morphants show difering phenotypes: they suggest that more time is needed for compensation, mutants having enough but not morphants

These investigators suggest a reason their mutants and their morphants show different phenotypes. The mutant embryos have had time enough to compensate for the knockdowns, hiding aspects of the loss-of-funciton phenotype, while the acute knockdown of the Morpholino shows a phenotype during time insufficient for compensation.

Yang C-R, Rajkovic G, Daldello EM, Luong XG, Chen J, Conti M. The RNA binding protein DAZL functions as repressor and activator of maternal mRNA translation during oocyte maturation. bioRxiv. 2019;[preprint] doi:10.1101/598805

Wednesday, May 15, 2019 - 10:28
Antisense oligonucleotides to treat spinal muscular atrophy (Shift Pharmaceuticals)

Antisense oligonucleotides to treat spinal muscular atrophy
Shift Pharmaceuticals (University of Missouri)
Abstract for Midwest Drug Development Conference, Sept. 30-Oct. 1, 2019. Omaha NE.

Monday, May 6, 2019 - 10:13
Morpholinos: Research Tools and Therapeutics (Cell Press Selections)

New Cell Press Selections: Morpholinos: Research Tools and Therapeutics

Friday, April 26, 2019 - 14:07
Storage of Morpholinos: refrigerate or room temperature?

Morpholino oligos are completely chemically & biologically (enzymatically) stable sitting in water at 4C or at room temperature as long as the pH is not extreme. However, they undergo physical changes that can affect their biological activity. Chilled storage increases the tendency of the oligos to associate with container walls (this is especially a problem if the oligos undergo freeze-thaw cycles). Once a Morpholino has associated tightly with the wall of a container, it can be difficult or impossible to get the oligo back into solution at neutral pH.

Tuesday, April 23, 2019 - 08:01
Proposed mechanism underlying genetic compensation

Genetic paradox explained by nonsense. Wilkinson MF. Nature. 2019;568:179-180. doi: 10.1038/d41586-019-00823-5

Genetic compensation triggered by mutant mRNA degradation. El-Brolosy MA et al. Nature. 2019;568:193-197. doi:10.1038/s41586-019-1064-z

Thursday, April 11, 2019 - 13:46
Antibody validation by Morpholino

Here is an example of using Morpholinos for antibody validation.

Ratzan W, Rayaprolu V, Killian SE, Bradley R, Kohout SC. The voltage sensing phosphatase (VSP) localizes to the apical membrane of kidney tubule epithelial cells. PLoS ONE. 2019;14(4):e0209056. doi:10.1371/journal.pone.0209056

Wednesday, April 10, 2019 - 12:44