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Fmn2 regulates growth cone motility by mediating a molecular clutch to generate traction forces

Authors: 
Ghate K, Mutalik SP, Sthanam LK, Sen S, Ghose A
Citation: 
bioRxiv. 2020;[preprint] doi:10.1101/2020.02.21.959759
Abstract: 
Growth cone - mediated axonal outgrowth and accurate synaptic targeting are central to brain morphogenesis. Translocation of the growth cone necessitates mechanochemical regulation of cell - extracellular matrix interactions and the generation of propulsive traction forces onto the growth environment. However, the molecular mechanisms subserving force generation by growth cones remain poorly characterized. The formin family member, Fmn2, has been identified earlier as a regulator of growth cone motility. Here, we explore the mechanisms underlying Fmn2 function in the growth cone. Using multiple components of the adhesion complexes, we show that Fmn2 regulates point contact stability. Analysis of F-actin retrograde flow reveals that Fmn2 functions as a clutch molecule and mediates the coupling of the actin cytoskeleton to the growth substrate, via the point contact adhesion complex. Using traction force microscopy, we show that the Fmn2-mediated clutch function is necessary for the generation of traction stresses by neurons. Our findings suggest that Fmn2, a protein associated with neurodevelopmental and neurodegenerative disorders, is a key regulator of a molecular clutch activity and consequently motility of neuronal growth cones.
Epub: 
Not Epub
Organism or Cell Type: 
Gallus gallus (chick) spinal cord explants
Delivery Method: 
electroporation