Guanidinium-linked morpholino-siRNA chimera: synthesis, biophysical properties and in vitro activity

This study introduces guanidinium morpholino RNA (GMO-RNA), an RNA analogue with enhanced cell permeability, nuclease resistance and RNA interference activity. Self-penetrating GMO-RNA chimeras leverage guanidinium-mediated endocytic uptake across multiple cell lines. Thermal studies validated duplex stability, and Eg5-targeting GMO-siRNA achieved effective gene silencing in cervical cancer cells, demonstrating therapeutic potential.