XPteg (Xenopus Proximal Tubules-Expressed Gene) Is Essential for Pronephric Mesoderm Specification and Tubulogenesis

Retinoic acid (RA) signaling is important for the early steps of nephrogenic cell fate specification. Here, we report a novel target gene of RA signaling named XPteg (Xenopusproximal tubules-expressed gene) which is critical for pronephric development. XPteg starts to be expressed at the earliest stage of embryonic kidney specification and was restricted to the pronephric proximal tubules during kidney development. Anti-sense morpholino (MO)-mediated knockdown of XPteg perturbed formation of pronephros as demonstrated by reduced expression of pronephric tubule markers. Conversely, overexpression of XPteg promoted endogenous and ectopic expression of those markers and expanded pronephric tubules. Treatment of retinoic acid induced the expression of XPteg in the pronephric field without protein synthesis. Furthermore, we found that the pronephric defects caused by a dominant negative RA receptor could be rescued by coexpression of XPteg. Taken together, these results suggest that XPteg functions as a direct transcriptional target of RA signaling to regulate pronephric tubulogenesis in Xenopus early development.