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|A nucleic acid binder, poor at binding MO||
Sen D, Patel G, Patel SS. Homologous DNA strand exchange activity of the human mitochondrial DNA helicase TWINKLE. Nucl Acids Res. 2016;[Epub ahead of print] doi:10.1093/nar/gkw098
DNA binds in the protein TWINKLE, but Morpholino is very poor at that binding. No surprise, it's likely due to lack of charge, but this is a nice published example of the low-binding characteristic.
|Wednesday, February 17, 2016 - 09:42|
|Antisense oligonucleotide-directed inhibition of nonsense-mediated mRNA decay||
Here's an interesting technique: inhibition of nonsense-mediated decay with oligos. I don't think the antisense oligos were Morpholinos.
Antisense oligonucleotide-directed inhibition of nonsense-mediated mRNA decay.
|Tuesday, February 2, 2016 - 10:24|
|Use of Morpholinos to Regulate Gene Expression in the Brain||
Here is a review of tools to manipulate gene expression in the brain. Just before the Conclusion is the section "Use of Morpholinos to Regulate Gene Expression in the Brain".
Walters BJ, Azam AB, Gillon CJ, Josselyn SA, Zovkic IB. Advanced In vivo Use of CRISPR/Cas9 and Anti-sense DNA Inhibition for Gene Manipulation in the Brain. Front Genet. 2016. doi:10.3389/fgene.2015.00362
|Monday, January 25, 2016 - 11:18|
|Morpholino-driven gene editing: A new horizon for disease treatment and prevention||
Though I did in the early days of commercial Morpholinos, now I generally don't enter review articles into the Morpholino database. This one came along and I want to keep a record of it, particularly for the list of diseases in Table 1. So, it goes here.
Subbotina E, Koganti SR, Hodgson-Zingman D, Zingman LV. Morpholino-driven gene editing: A new horizon for disease treatment and prevention. Clin Pharmacol Ther. 2015 Oct 16. doi: 10.1002/cpt.276. [Epub ahead of print]
|Thursday, December 17, 2015 - 13:44|
|Example of splice enhancer targeting||
Liquori A, Vaché C, Baux D, Blanchet C, Hamel C, Malcolm S, Koenig M, Claustres M, Roux AF. Whole USH2A Gene Sequencing Identifies Several New Deep Intronic Mutations. Hum Mutat. 2015 Nov 2. doi: 10.1002/humu.22926. [Epub ahead of print]
|Thursday, December 3, 2015 - 09:40|
|Bathing zebrafish embryos with Vivo-Morpholinos||
Wong TT, Zohar Y. Production of reproductively sterile fish by a non-transgenic gene silencing technology. Sci Rep. 2015 Oct 29;5:15822. doi: 10.1038/srep15822.
This group bathed zebrafish embryos (with chorion intact) in Vivo-Morpholino solutions, with oligos targeting dead-end (dnd).
|Monday, November 2, 2015 - 10:09|
|Genetic compensation induced by deleterious mutations but not gene knockdowns||
When assessing gene function, gene knockdown reveals what can be hidden by compensation in mutants
Rossi A, Kontarakis Z, Gerri C, Nolte H, Hölper S, Krüger M, Stainier DYR. Genetic compensation induced by deleterious mutations but not gene knockdowns. Nature. 2015;[Epub ahead of print] doi:10.1038/nature14580
|Monday, July 13, 2015 - 11:15|
|Morpholinos to validate target specificity of antibodies||
Here's an example of using Morpholinos to validate target specificity of antibodies.
Belgacem YH, Borodinsky LN. Inversion of Sonic hedgehog action on its canonical pathway by electrical activity. Proc Natl Acad Sci U S A. 2015 Mar 31;112(13):4140-5. doi: 10.1073/pnas.1419690112. Epub 2015 Mar 17.
|Friday, June 12, 2015 - 10:54|
|Websites (and some papers) about morphant and mutant comparisons||
I've been watching several websites with discussions of morphant and mutant comparisons. Here are links to the sites. The comments sections have been very supportive of Morpholino technology. I'll update this page as I find more.
|Thursday, March 5, 2015 - 13:23|
|Morpholinos, mutants, and phenocopying||
I was concerned when Stefan Schulte-Merker and Didier Stainier' paper appeared in mid-2014, first in Development and then on The Node. Later in 2014 Nathan Lawson's paper in Developmental Cell was published. Prior to these papers I was intrigued and excited by CRISPR technology but felt that, while it might temporarily displace some use of Morpholinos, in the long run it would benefit Morpholino use by making the zebrafish model stronger.
|Wednesday, February 11, 2015 - 15:18|