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|Knockdowns in mosquito guts||
Vivo-Morpholinos were fed to mosquitos. The oligos decreased the amount of MAPK protein in the midgut and the number of Plasmodium oocysts in the mudgut.
"The feeding protocol described in the present study provides several advantages over current methods for gene knockdown in insects. The method is easy to apply, requires no special skills for delivery, and is highly target specific. ... This protocol should therefore be considered as an efficient alternative in studies requiring target specific protein knockdown in mosquitoes and other arthropods."
|Thursday, May 29, 2014 - 11:45|
|Morpholino electroporated into human T-cells induces alternative splicing without interferon response||
Michel M, Wilhelmi I, Schultz A-S, Preussner M, Heyd F. Activation-induced Tumor Necrosis Factor Receptor-associated Factor 3 (Traf3) Alternative Splicing Controls the Noncanonical Nuclear Factor κB Pathway and Chemokine Expression in Human T Cells. J Biol Chem. 2014;289:13651-60. doi:10.1074/jbc.M113.526269
"Furthermore, the interferon response, which can be regulated by Traf3 (35), was not altered in Traf3E8 MO-treated cells (data not shown)" supports that the Morpholino did not induce an innate immune response.
|Friday, May 9, 2014 - 09:21|
|Vivo-Morpholino in rat brain||Shen H-w, Scofield MD, Boger H, Hensley M, Kalivas PW. Synaptic Glutamate Spillover Due to Impaired Glutamate Uptake Mediates Heroin Relapse. J Neurosci. 2014;34(16):5649-57 doi:10.1523/JNEUROSCI.4564-13.2014 http://www.jneurosci.org/content/34/16/5649.abstract||Thursday, April 17, 2014 - 09:16|
|Nanoparticle activated by near-IR delivers and cleaves Photo-Morpholino||Jayakumar MK, Bansal A, Huang K, Yao R, Li BN, Zhang Y. Near-Infrared-Light-Based Nano-Platform Boosts Endosomal Escape and Controls Gene Knockdown in Vivo. ACS Nano. 2014 Apr 14. [Epub ahead of print] http://www.cell.com/current-biology/abstract/S0960-9822%2812%2901327-9||Wednesday, April 16, 2014 - 13:34|
|Using a GFP reporter to test a knockdown||Romaker D, Kumar V, Cerqueira DM, Cox RM, Wessely O. MicroRNAs are critical regulators of tuberous sclerosis complex and mTORC1 activity in the size control of the Xenopus kidney. Proc Natl Acad Sci U S A. 2014 Apr 14. [Epub ahead of print] The authors report several test constructs in which their upstream Morpholino target is ligated to a GFP coding sequence and then tested for its response to a Morpholino translation blocker. See figure S6 in the supporting online information.||Wednesday, April 16, 2014 - 12:59|
|Jon's blog for notes on new applications and pointers to techniques||Welcome! I'll be keeping notes here on citations that demonstrate techniques that catch my interest using Morpholino oligos.||Wednesday, April 16, 2014 - 12:00|