Using a GFP reporter to test a knockdown
Romaker D, Kumar V, Cerqueira DM, Cox RM, Wessely O. MicroRNAs are critical regulators of tuberous sclerosis complex and mTORC1 activity in the size control of the Xenopus kidney. Proc Natl Acad Sci U S A. 2014 Apr 14. [Epub ahead of print]
The authors report several test constructs in which their upstream Morpholino target is ligated to a GFP coding sequence and then tested for its response to a Morpholino translation blocker. See figure S6 in the supporting online information.
Wednesday, April 16, 2014 - 12:59